Vasn ELISA Kit
Quick Overview for Vasn ELISA Kit (ABIN2044787)
Target
See all Vasn ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.060 ng/mL
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Vasorin (Vasn) ELISA Kit
Vasn Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Vasorin (Vasn) ELISA KitVasn Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Vasorin (VASN) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vasorin (VASN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vasorin (VASN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vasorin (VASN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vasorin (VASN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- Vasn (Vasorin (Vasn))
Target See all Vasn ELISA Kits
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