VAMP2 ELISA Kit

Catalog No. ABIN2044789

Human VAMP2 ELISA Kit, Colorimetric assay for quantification of Human VAMP2.

Quick Overview for VAMP2 ELISA Kit (ABIN2044789)

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details VAMP2 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.057 ng/mL

Application Details

Plate: Pre-coated

Protocol: Vesicle Associated Membrane Protein 2 (VAMP2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vesicle Associated Membrane Protein 2 (VAMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vesicle Associated Membrane Protein 2 (VAMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vesicle Associated Membrane Protein 2 (VAMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vesicle Associated Membrane Protein 2 (VAMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for VAMP2

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Alternative Name: Vesicle Associated Membrane Protein 2 (VAMP2)

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport