MAVS ELISA Kit

Catalog No. ABIN2044795

Human MAVS ELISA Kit Colorimetric assay for quantification of Human MAVS.

Quick Overview for MAVS ELISA Kit (ABIN2044795)

Target: MAVS (Mitochondrial Antiviral Signaling Protein (MAVS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details MAVS ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.068 ng/mL

Application Details

Plate: Pre-coated

Protocol: Virus Induced Signaling Adapter (VISA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Virus Induced Signaling Adapter (VISA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Virus Induced Signaling Adapter (VISA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Virus Induced Signaling Adapter (VISA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Virus Induced Signaling Adapter (VISA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MAVS

Target: MAVS (Mitochondrial Antiviral Signaling Protein (MAVS))

Alternative Name: Virus Induced Signaling Adapter (VISA)

Pathways: Activation of Innate immune Response, Inositol Metabolic Process, Hepatitis C