Vitronectin ELISA Kit

Catalog No. ABIN2044801

Mouse Vitronectin ELISA Kit Colorimetric assay for quantification of Mouse Vitronectin.

Quick Overview for Vitronectin ELISA Kit (ABIN2044801)

Target: Vitronectin (VTN)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6.25-400 ng/mL

Application: ELISA

Product Details Vitronectin ELISA Kit

Minimum Detection Limit: 6.25 ng/mL

Sensitivity: < 2.59 ng/mL

Application Details

Plate: Pre-coated

Protocol: Vitronectin (VTN) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitronectin (VTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitronectin (VTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitronectin (VTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vitronectin (VTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Vitronectin

Target: Vitronectin (VTN)

Pathways: Autophagy, Smooth Muscle Cell Migration