WIF1 ELISA Kit

Catalog No. ABIN2044847

This Colorimetric ELISA kit is designed for the quantitative measurement of Human WIF1.

Quick Overview for WIF1 ELISA Kit (ABIN2044847)

Target: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details WIF1 ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Sensitivity: < 6.9 pg/mL

Application Details

Plate: Pre-coated

Protocol: WNT Inhibitory Factor 1 (WIF1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT Inhibitory Factor 1 (WIF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT Inhibitory Factor 1 (WIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT Inhibitory Factor 1 (WIF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of WNT Inhibitory Factor 1 (WIF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for WIF1

Target: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation