WWP2 ELISA Kit

Catalog No. ABIN2044855

Human WWP2 ELISA Kit Colorimetric assay for quantification of Human WWP2.

Quick Overview for WWP2 ELISA Kit (ABIN2044855)

Target: WWP2 (WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details WWP2 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.27 ng/mL

Application Details

Plate: Pre-coated

Protocol: WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for WWP2

Target: WWP2 (WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2))

Pathways: Negative Regulation of Transporter Activity