YBX1 ELISA Kit

Catalog No. ABIN2044859

This Colorimetric ELISA kit is designed for the quantitative measurement of Human YBX1.

Quick Overview for YBX1 ELISA Kit (ABIN2044859)

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details YBX1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Brand: BioAssay™

Sensitivity: < 0.064 ng/mL

Application Details

Plate: Pre-coated

Protocol: Y-Box Binding Protein 1 (YBX1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Y-Box Binding Protein 1 (YBX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Y-Box Binding Protein 1 (YBX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Y-Box Binding Protein 1 (YBX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Y-Box Binding Protein 1 (YBX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for YBX1

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Alternative Name: Y-Box Binding Protein 1 (YBX1)

Pathways: Regulation of Muscle Cell Differentiation