beta-2 Microglobulin ELISA Kit
Quick Overview for beta-2 Microglobulin ELISA Kit (ABIN2111978)
Target
See all beta-2 Microglobulin (B2M) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 4.7 ng/mL
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Analytical Method
- Quantitative
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Sensitivity
- < 2.1 ng/mL
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Components
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A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL
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beta-2-Microglobulin (B2M) ELISA Kit
B2M Reactivity: Human Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Plasma, Serum, Urine
beta-2-Microglobulin (B2M) ELISA KitB2M Reactivity: Human Colorimetric Competition ELISA 0.025-10 μg/mL Serum, Urine
beta-2-Microglobulin (B2M) ELISA KitB2M Reactivity: Dog Colorimetric Sandwich ELISA 0.78 μg/mL - 50 μg/mL Plasma, Serum, Tissue Homogenate
beta-2-Microglobulin (B2M) ELISA KitB2M Reactivity: Mouse Colorimetric Sandwich ELISA 0.41 ng/mL - 300 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
beta-2-Microglobulin (B2M) ELISA KitB2M Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
beta-2-Microglobulin (B2M) ELISA KitB2M Reactivity: Human Colorimetric Sandwich ELISA 3.125 ng/mL - 100 ng/mL Plasma, Serum, Urine
beta-2-Microglobulin (B2M) ELISA KitB2M Reactivity: Human Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
beta-2-Microglobulin (B2M) ELISA KitB2M Reactivity: Human Colorimetric Competition ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Plasma, Serum
beta-2-Microglobulin (B2M) ELISA KitB2M Reactivity: Monkey Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
beta-2-Microglobulin (B2M) ELISA KitB2M Reactivity: Monkey Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Urine
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Plate
- Pre-coated
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Protocol
- The Mouse beta-2-Microglobulin (b2M) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of b2M in mouse serum, plasma, urine, tissue homogenates, cell lysates, cell culture supertantants and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to b2M. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to b2M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain b2M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of b2M in the sample is then determined by comparing the O.D. of the sample to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
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Alternative Name
- beta-2 Microglobulin (b2M)
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Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Target See all beta-2 Microglobulin (B2M) ELISA Kits
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