Granulin ELISA Kit

Catalog No. ABIN2111984

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Granulin.

Quick Overview for Granulin ELISA Kit (ABIN2111984)

Target: Granulin (GRN)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.06-4 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details Granulin ELISA Kit

Minimum Detection Limit: 0.06 ng/mL

Analytical Method: Quantitative

Sensitivity: 32 pg/mL

Components: Plate coated with human progranulin antibody, 1x96wells
Wash Buffer 10X, 1x50 mL
Diluent 5X, 1x50 mL
Detection Antibody, 1x10 mL
Detector 100X (HRP Labeled Streptavidin), 1x150 µL
Human progranulin Standard, lyophilized, 1x8 ng
Human progranulin QC sample, lyophilized, 1x vial
TMB Substrate Solution, 1x10 mL
Stop Solution, 1x10 mL
Plate sealers, 3x plastic film

Application Details

Plate: Pre-coated

Protocol: This BioAssay-is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3-,5,5--tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of progranulin in the samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Granulin

Target: Granulin (GRN)

Alternative Name: Progranulin

Target Type: Viral Protein

Background: Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. New cutting-edge publications have shown that progranulin might be a biomarker not only for FTLD, but also for other types of Alzheimer-s disease (AD). This new data even supposes the potential of progranulin to be a biomarker for MCI (Mild Cognitive Impairment), which would be a hallmark in AD research. Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis.