EPI ELISA Kit

Catalog No. ABIN2648480

This Colorimetric ELISA kit is designed for the quantitative measurement of Chemical EPI.

Quick Overview for EPI ELISA Kit (ABIN2648480)

Target: EPI (Epinephrine/Adrenaline (EPI))

Reactivity: Chemical

Detection Method: Colorimetric

Application: ELISA

Sample Type: Urine, Plasma

Product Details EPI ELISA Kit

Sensitivity: Urine: 0.16 ng/ml, Plasma: 8 pg/ml

Characteristics: Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitters, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 15 μL

Plate: Pre-coated

Protocol: The competitive Adrenaline (Epinephrine) ELISA Kit uses the microtiter plate format. Adrenaline is bound to the solid phase of the microtiter plate. Acylated adrenaline from the sample and solid phase bound adrenaline compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase adrenaline is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase adrenaline is inversely proportional to the adrenaline concentration of the sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for EPI

Target: EPI (Epinephrine/Adrenaline (EPI))

Alternative Name: Adrenaline (Epinephrine)

Target Type: Chemical