IL4 Receptor ELISA Kit

Catalog No. ABIN2648537

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL4 Receptor.

Quick Overview for IL4 Receptor ELISA Kit (ABIN2648537)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Product Details IL4 Receptor ELISA Kit

Purpose: Human IL-4R ELISA is to be used for the in-vitro quantitative determination of soluble IL-4 receptor molecule (CD124) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human IL-4R.

Analytical Method: Quantitative

Characteristics: Human IL-4R ELISA Assay kit is for Research Use Only.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: The IL-4R Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-4R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4R concentrations and unknowns are pipetted into these wells. During the first incubation, the IL-4R antigen and a biotinylated monoclonal antibody specific for IL-4R are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-4R present in the samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: CD124 / IL-4R

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process