Syndecan 1 ELISA Kit

Catalog No. ABIN2648545

Human Syndecan 1 ELISA Kit Colorimetric assay for quantification of Human Syndecan 1.

Quick Overview for Syndecan 1 ELISA Kit (ABIN2648545)

Target: Syndecan 1 (SDC1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details Syndecan 1 ELISA Kit

Purpose: sCD138 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of sCD138 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human sCD138.

Analytical Method: Qualitative and Quantitative

Sensitivity: 2.56 ng/ml

Characteristics: Human sCD138 ELISA Assay kit is for Research Use Only.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: A capture Antibody highly specific for CD138 has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of CD138 samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-CD138 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of CD138 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of CD138 in any sample tested.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Syndecan 1

Target: Syndecan 1 (SDC1)

Alternative Name: CD138 / SYNDECAN-1

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism