Thrombomodulin ELISA Kit

Catalog No. ABIN2648546

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Thrombomodulin.

Quick Overview for Thrombomodulin ELISA Kit (ABIN2648546)

Target: Thrombomodulin (THBD)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details Thrombomodulin ELISA Kit

Purpose: sCD141 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of sCD141 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human sCD141.

Analytical Method: Qualitative and Quantitative

Sensitivity: 0.31 ng/ml

Characteristics: Human sCD141 ELISA Assay kit is for Research Use Only.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 1 - 2 h

Plate: Pre-coated

Protocol: A capture Antibody highly specific for CD141 has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of CD141 samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-CD141 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of CD141 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of CD141 in any sample tested.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Thrombomodulin

Target: Thrombomodulin (THBD)

Alternative Name: CD141 / THROMBOMODULIN