CD44 ELISA Kit

Catalog No. ABIN2648547

This Human CD44 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CD44.

Quick Overview for CD44 ELISA Kit (ABIN2648547)

Target: CD44

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Product Details CD44 ELISA Kit

Purpose: CD44 ELISA is to be used for the in-vitro quantitative determination of soluble CD44 (CD44/phagocytic glycoprotein-1/Pgp-1) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD44.

Analytical Method: Quantitative

Characteristics: Human CD44 ELISA Assay kit

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: The CD44 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for CD44 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD44 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD44 antigen, standards and zeros are bound this is followed by a separate incubation where the biotinylated monoclonal antibody specific for CD44 is added. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD44 present in the samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for CD44

Target: CD44

Alternative Name: CD44 / Pgp-1

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling