CD86 ELISA Kit

Catalog No. ABIN2648551

Human CD86 ELISA Kit Colorimetric assay for quantification of Human CD86.

Quick Overview for CD86 ELISA Kit (ABIN2648551)

Target: CD86

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details CD86 ELISA Kit

Purpose: Human CD86 ELISA is to be used for the in-vitro quantitative determination CD86 in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD86.

Analytical Method: Quantitative

Sensitivity: 0.6 U/ml

Characteristics: Human CD86 ELISA Assay kit is for Research Use Only.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The CD86 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD86 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD86 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD86 antigen and a biotinylated monoclonal antibody specific for CD86 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD86 present in the samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for CD86

Target: CD86

Alternative Name: CD86

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation