Chromogranin A ELISA Kit

Catalog No. ABIN2648557

Human Chromogranin A ELISA Kit Colorimetric assay for quantification of Human Chromogranin A.

Quick Overview for Chromogranin A ELISA Kit (ABIN2648557)

Target: Chromogranin A (CHGA)

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma

Product Details Chromogranin A ELISA Kit

Sensitivity: 2 ng/ml

Characteristics: Human Chromogranin A ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Chromogranin A levels in EDTA-plasma and serum samples. This assay exclusively measures human Chromogranin A without the high dose "hook" effect up to 1,000,000 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 15 μL

Assay Time: 2.5 h

Protocol: Human Chromogranin A ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human chromogranin A in EDTA-plasma or serum sample. The assay utilizes the two-site "sandwich" technique with two selected antibodies that bind to different epitopes of human Chromogranin A. Assay standards, controls and patient samples are added directly to wells of microplate that is coated with a polyclonal chromogranin A antibody. After the first incubation period, the antibody on the wall of microtiter well captures human chromogranin A in the sample and unbound proteins in each microtiter well is washed away. Then a horseradish peroxidase (HRP) labeled monoclonal anti-human chromogranin A antibody is added to each microtiter well and a "sandwich" of "monoclonal antibody - human chromogranin A - polyclonal antibody" is formed. The unbound monoclonal antibody is removed in the subsequent washing step. For the detection of this immunocomplex, the well is incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the chromogranin A on the wall of the microtiter well is directly proportional to the amount of chromogranin A in the sample. A standard curve is generated by plotting the absorbance versus the respective human chromogranin A concentration for each standard with a 4 parameter curve fit. The concentration of human chromogranin A in test samples is determined directly from this standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: Chromogranin A

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling