CRP ELISA Kit
Quick Overview for CRP ELISA Kit (ABIN2648561)
Target
See all CRP ELISA KitsReactivity
Detection Method
Application
Sample Type
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Sensitivity
- 0.12 mg/dL
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Characteristics
- C-Reactive Protein (CRP) has been demonstrated to be a general indicator of major tissue damage. Hence, studies have shown that CRP can be used to indicate a stroke or heart attack because major blood vessels leading to the heart or brain are damaged and release large quantities of CRP during these disease states. CRP is a particularly useful indicator of CHD in women and in patients that demonstrate no other plasma circulating biochemical indicators.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 10 μL
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Assay Time
- 2.5 h
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Plate
- Pre-coated
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Protocol
- The C-Reactive Protein (CRP) ELISA assay kit determines total human CRP according to the "sandwich" principle. CRP in samples and standards binds to antibodies which are coated to the microtiter plate. After a washing step a peroxidase labeled detection antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by the addition of an acidic stop solution. The optical densities are read at 450 nm in a microtiter plate reader. The CRP concentration can be calculated from the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C,-20 °C
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- CRP (C-Reactive Protein (CRP))
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Pathways
- Carbohydrate Homeostasis
Target See all CRP ELISA Kits
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