EGF ELISA Kit

Catalog No. ABIN2648643

This Human EGF ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human EGF.

Quick Overview for EGF ELISA Kit (ABIN2648643)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details EGF ELISA Kit

Purpose: Human Epidermal Growth Factor (EGF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Epidermal Growth Factor (EGF) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Characteristics: Human Epidermal Growth Factor (EGF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: Human Epidermal Growth Factor (EGF) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for EGF is added to the wells and binds to the combination of capture antibody- EGF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of EGF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven EGF standard dilutions and EGF sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation