Folic Acid ELISA Kit
Quick Overview for Folic Acid ELISA Kit (ABIN2648657)
Target
See all Folic Acid (FA) ELISA KitsReactivity
Detection Method
Application
Sample Type
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Sensitivity
- 2 ng/ml
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Characteristics
- Folates play an important role in the synthesis of nucleic acids and some amino acids and gained re-cently increased interest because they belong to the group of antioxidative vitamins. In the last years the in-fluence of folic acid supplementation to avoid abortion and dysraphism was a topic of research increasingly. Folic acid as the most stable representative of the group of folates is added to a broad range of food.
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Folic Acid (FA) ELISA Kit
FA Reactivity: Various Species Colorimetric Competition ELISA 39.1 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Folic Acid (FA) ELISA KitFA Reactivity: Cow Colorimetric Competition ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Folic Acid (FA) ELISA KitFA Reactivity: Human Colorimetric Competition ELISA 12.5 pg/mL - 200 pg/mL Plasma, Serum
Folic Acid (FA) ELISA KitFA Reactivity: Mouse Colorimetric 0.195-50 ng/mL Plasma, Serum
Folic Acid (FA) ELISA KitFA Reactivity: Various Species Colorimetric Sandwich ELISA 0.312-20 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Folic Acid (FA) ELISA KitFA Reactivity: Rat Colorimetric 19.5-5000 pg/mL Plasma, Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Assay Time
- 2 h
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Plate
- Pre-coated
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Protocol
- The Folic Acid ELISA Assay kit is a quantitative assay based on the principle of the enzyme linked immu-nosorbent assay. A folic acid conjugate is bound on the surface of a microtiter plate. Folic acid containing samples or standards and an antibody directed against folic acid are given into the wells of the microtiter plate. Immobi-lized and free folic acid compete for the antibody binding sites. After one hour incubation at room tem-perature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugate against the antibody is given into the wells and after another hour incubation, the plate is washed again. Then a substrate solution is added and incubated for 20 minutes, resulting in the devel-opment of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of folic acid is indirectly proportional to the colour inten-sity of the test sample.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- Folic Acid (FA)
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Alternative Name
- Folic Acid
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Target Type
- Chemical
Target See all Folic Acid (FA) ELISA Kits
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