GLP-1 ELISA Kit

Catalog No. ABIN2648663

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse and Rat GLP-1.

Quick Overview for GLP-1 ELISA Kit (ABIN2648663)

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Reactivity: Mouse, Rat

Detection Method: Colorimetric

Application: ELISA

Sample Type: Plasma

Product Details GLP-1 ELISA Kit

Sensitivity: 0.1 pM

Characteristics: Mouse/Rat Active GLP-1 ELISA Assay Kit (enzyme-linked immunoassay kit) is intended quantitative determination of bioactive glucagon-like peptide-1 (7-36) amide [GLP-1 (7-36)] level in rat and mouse plasma samples with only 20 μL of sample volume. E The primary amino acid sequence of GLP-1 peptide is identical among mammalian species, i.e. rat, mouse, pig, human, etc. Eagle Biosciences Mouse/Rat Active GLP-1 ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Assay Time: 1 d

Protocol: Mouse/Rat Active GLP-1 ELISA Assay Kit is designed, developed and produced for the quantitative measurement of bioactive GLP-1 (7-36) in rodent plasma that usually have limited amount of sample available for analysis. The assay utilizes the two-site "sandwich" technique with two selected GLP-1 (7-36) specific antibodies. Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 (7-36) specific antibody and a horseradish peroxidase (HRP) conjugated GLP-1 (7-36) specific antibody is added to each well. After the first incubation period, a "sandwich" immunocomplex of "Streptavidin - Biotin-Antibody - GLP-1(7-36) - HRP conjugated antibody" is formed and attached to the wall of the plate. The unbound HRP conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36) on the wall of the microtiter well is directly proportional to the amount of GLP-1 (7-36) in the sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for GLP-1

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Alternative Name: GLP-1