GLP-1 ELISA Kit

Catalog No. ABIN2648664

This Human, Mouse and Rat GLP-1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human, Mouse and Rat GLP-1.

Quick Overview for GLP-1 ELISA Kit (ABIN2648664)

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Binding Specificity: total

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Application: ELISA

Sample Type: Plasma

Product Details GLP-1 ELISA Kit

Sensitivity: 0.6 pmol/L

Characteristics: The primary amino acid sequence of GLP-1 peptide is identical among mammalian species, i.e. rat, mouse, pig, human, etc. GLP-1 Total ELISA Assay Kit (enzyme-linked immunoassay kit) is intended quantitative determination of sum value of glucagon-like peptide-1 (7-36) and (9-36) [GLP-1 (7-36)] and (9-36) [GLP-1 (9-36)] in plasma samples. Eagle Biosciences GLP-1 Total ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Protocol: Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 specific antibody and a horseradish peroxidase (HRP)-conjugated GLP-1 specific antibody is added to each well. After the first incubation period, a "sandwich" immunocomplex of "Streptavidin - Biotin-Antibody - GLP-1(7-36)/(9-36) - HRP-conjugated antibody" is formed and attached to the wall of the plate. The unbound HRP-conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36)/(9-36) on the wall of the microtiter well is directly proportional to the amount of Total GLP-1 in the sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for GLP-1

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Alternative Name: GLP-1