HAMA ELISA Kit

Catalog No. ABIN2648666

This Colorimetric ELISA kit is designed for the quantitative measurement of Human and Mouse HAMA.

Quick Overview for HAMA ELISA Kit (ABIN2648666)

Target: HAMA (Human Anti-Mouse Antibody (HAMA))

Reactivity: Human, Mouse

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma

Product Details HAMA ELISA Kit

Sensitivity: 2 ng/ml

Characteristics: Human Anti-Mouse Antibody (HAMA) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Anti-Mouse Antibody (HAMA) levels in serum. Human Anti-Mouse Antibody (HAMA) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 25 μL

Assay Time: 2 h

Protocol: Human Anti-Mouse Antibody (HAMA) ELISA Assay Kit is designed, developed and produced for the quantitative measurement of HAMA in serum and plasma samples. The assay utilizes the two-site "sandwich" technique with two selected antibodies that bind to HAMA. Assay standards, controls and patient samples are directly added to wells of a microplate that is coated with murine IgG. After the first incubation period, the HAMA binds to the murine IgG on the wall of microtiter well and unbound proteins in each microtiter well are washed away. Then a horseradish peroxidase (HRP) labeled murine IgG is added to each microtiter well and a "sandwich" of "murine IgG HAMA - murine IgG" is formed. The unbound HRP conjugated murine IgG is removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to HAMA on the wall of the microtiter well is directly proportional to the amount of HAMA in the sample. A standard curve is generated by plotting the absorbance versus the respective HAMA concentration for each standard on point-to-point, cubical scales or 4 parameter curve fit. The concentration of HAMA in test samples is determined directly from this standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for HAMA

Target: HAMA (Human Anti-Mouse Antibody (HAMA))

Alternative Name: HAMA Antibody

Target Type: Antibody