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Histamine ELISA Kit

Name: Histamine ELISA Kit
SKU: ABIN2648672
Price: 1024.62 USD
Availability: InStock

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Histamine.

Catalog No. ABIN2648672

Quick Overview for Histamine ELISA Kit (ABIN2648672)

Target

See all Histamine (HIS) ELISA Kits

Histamine (HIS)

Reactivity

Human

Detection Method

Colorimetric

Application

ELISA

Sample Type

Plasma, Urine, Cell Culture Cells

Discover our top product HIS ELISA Kit

Sensitivity

0.02 ng/mL

Characteristics

Histamine plays a major role in the initial phase of an anaphylactic reaction. The quantification of histamine in plasma after allergen administration is of clinical interest. Histamine is part of the immune response to foreign pathogens and it increases the permeability of the capillaries to white blood cells and other proteins in order to allow them to engage foreign invaders in the affected tissues. Responsible for the biological effects of histamine in tissue are the activation of different surface receptors, for instance H1, H2 and H3. Histamine is involved in the regulating physiological function in the gut and acting as a neurotransmitter.
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Application Notes

Optimal working dilution should be determined by the investigator.

Sample Volume

50 μL

Assay Time

2.5 h

Plate

Pre-coated

Protocol

The competitive Histamine ELISA kit uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated histamine and solid phase bound histamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase histamine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase histamine is inversely proportional to the histamine concentration of the sample.

Restrictions

For Research Use only
Storage

4 °C
Target See all Histamine (HIS) ELISA Kits

Histamine (HIS)

Alternative Name

Histamine

Target Type

Chemical