Homoarginine ELISA Kit

Catalog No. ABIN2648674

Human ELISA Kit Colorimetric assay for quantification of Human .

Quick Overview for Homoarginine ELISA Kit (ABIN2648674)

Target: Homoarginine

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details

Sensitivity: 0.05 μmol/L

Characteristics: Homoarginine is a non-essential cationic amino acid, which is formed from lysine. In vitro and in vivo, homoarginine shows characteristics similar to arginine. Epidemiological investigations in two large independent cohorts, namely the German diabetes dialysis (4D) study and the Ludwigshafen Risk and Cardiovascular Health (LURIC) study have identified homoarginine as useful predictor of cardiovascular events and mortality.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Assay Time: 2.5 h

Plate: Pre-coated

Protocol: The competitive Homoarginine ELISA Assay Kit uses the microtiter plate format. Homoarginine is bound to the solid phase of the microtiter plate. Homoarginine in the samples is acylated and competes with solid phase bound Homoarginine for a fixed number of rabbit anti-Homoarginine antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase Homoarginine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase Homoarginine is inversely proportional to the Homoarginine concentration of the sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Homoarginine

Target Type: Amino Acid