ICAM1 ELISA Kit

Catalog No. ABIN2648681

This Colorimetric ELISA kit is designed for the quantitative measurement of Human ICAM1.

Quick Overview for ICAM1 ELISA Kit (ABIN2648681)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details ICAM1 ELISA Kit

Purpose: Human Intercellular Adhesion Molecule 1 (SICAM-1) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Intercellular Adhesion Molecule 1 (sICAM-1) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Sensitivity: 31 pg/ml

Characteristics: Human Intercellular Adhesion Molecule 1 (sICAM-1) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: Human Intercellular Adhesion Molecule 1 (sICAM-1) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for sICAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sICAM-1 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for sICAM-1 is added to the wells and binds to the combination of capture antibody- sICAM-1 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of sICAM-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven sICAM-1 standard dilutions and sICAM-1 sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: sICAM-1

Target Type: Viral Protein

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling