Interferon gamma ELISA Kit

Catalog No. ABIN2648682

This Mouse Interferon gamma ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse Interferon gamma.

Quick Overview for Interferon gamma ELISA Kit (ABIN2648682)

Target: Interferon gamma (IFNG)

Reactivity: Mouse

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details Interferon gamma ELISA Kit

Purpose: Mouse Interferon Gamma (IFN-γ) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interferon Gamma (IFN-γ) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Sensitivity: 7 pg/ml

Characteristics: Mouse Interferon Gamma (IFN-γ) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: Mouse Interferon Gamma (IFN-γ) ELISA Assay Kit the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IFN-γ is added to the wells and binds to the combination of capture antibody-IFN-γ in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IFN-γ present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IFN-γ standard dilutions and IFN-γ sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN-gamma

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy