Interferon gamma ELISA Kit

Catalog No. ABIN411283

Mouse Interferon gamma ELISA Kit, Colorimetric assay for quantification of Mouse Interferon gamma, that has been mentioned in 33+ publications.

Quick Overview for Interferon gamma ELISA Kit (ABIN411283)

Target: Interferon gamma (IFNG)

Binding Specificity: AA 23-155

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details Interferon gamma ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit to quantitate Mouse Ifng in cell culture supernatants and serum.

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: H23-C155

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 5 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.3%, 5.5%, 4.4%
Inter-Assay Precision (CV%): 4.4%, 5.5%, 4.4%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for Interferon gamma ELISA Kit (ABIN411283)

Qin, Gao, Liu, Liu, Wang, Zou: "Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment." in: Oncology letters, Vol. 15, Issue 2, pp. 1630-1638, (2018) (PubMed).

Lu, Liu, Jin, Chen, Liang, Qiu, Dai: "Herbal Components of a Novel Formula PSORI-CM02 Interdependently Suppress Allograft Rejection and Induce CD8+CD122+PD-1+ Regulatory T Cells." in: Frontiers in pharmacology, Vol. 9, pp. 88, (2018) (PubMed).

Hu, Feng, Wang, Song, Ni: "5-BDBD ameliorates an OVA-induced allergic asthma by the reduction of Th2 cytokines production." in: Iranian journal of basic medical sciences, Vol. 21, Issue 4, pp. 364-369, (2018) (PubMed).

Zhang, Li, Wang, Xie, Li, Zuo, Kong, Wang, Wang: "Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis." in: Frontiers in microbiology, Vol. 9, pp. 909, (2018) (PubMed).

Xu, Liu, Wang, Li, Wu, Li, Di, You, Jiang et al.: "3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress ..." in: Oxidative medicine and cellular longevity, Vol. 2018, pp. 3271617, (2018) (PubMed).

Liu, Huang, Yao, Yang: "The Immunotherapeutic Role of Bacterial Lysates in a Mouse Model of Asthma." in: Lung, (2017) (PubMed).

He, Liu, Xu: "The cryo-thermal therapy eradicated melanoma in mice by eliciting CD4(+) T-cell-mediated antitumor memory immune response." in: Cell death & disease, Vol. 8, Issue 3, pp. e2703, (2017) (PubMed).

Wang, Zhang, Wang, Gadahi, Xu, Yan, Song, Li: "Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1α) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis." in: Frontiers in microbiology, Vol. 8, pp. 168, (2017) (PubMed).

Liang, Song, Liang, Zhang, Gao, Fan: "The hemagglutinin-neuramidinase protein of Newcastle disease virus upregulates expression of the TRAIL gene in murine natural killer cells through the activation of Syk and NF-κB." in: PLoS ONE, Vol. 12, Issue 6, pp. e0178746, (2017) (PubMed).

Xu, Zhang, Li, Liu, Tang, Fan: "hsdS, Belonging to the Type I Restriction-Modification System, Contributes to the Streptococcus suis Serotype 2 Survival Ability in Phagocytes." in: Frontiers in microbiology, Vol. 8, pp. 1524, (2017) (PubMed).

Wang, Zhang, Xi, Huang, Nie, Zhou, Deng, Tao: "Establishment of a mouse model of lipopolysaccharide-induced neutrophilic nasal polyps." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5275-5282, (2017) (PubMed).

Wang, Su, Dong, Shi, Gao, Wang: "Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice." in: Human vaccines & immunotherapeutics, Vol. 10, Issue 4, pp. 1057-63, (2016) (PubMed).

Wu, Wen, Xiang, Huang, Gao, Qu: "Immunostimulative Activity of Low Molecular Weight Chitosans in RAW264.7 Macrophages." in: Marine drugs, Vol. 13, Issue 10, pp. 6210-25, (2016) (PubMed).

Wang, Wang, Sun, Zhang, Liu, Gadahi, Hassan, Xu, Yan, Song, Li: "Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii elongation factor 1-alpha." in: BMC infectious diseases, Vol. 15, pp. 448, (2016) (PubMed).

Wang, Ji, Zhang, Fan, Zhang, Shi, Zhou, Chen, Wang, Wang: "Stimulation of dendritic cells by DAMPs in ALA-PDT treated SCC tumor cells." in: Oncotarget, Vol. 6, Issue 42, pp. 44688-702, (2016) (PubMed).

Chen, Zhang, Sun: "Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica." in: BMC complementary and alternative medicine, Vol. 15, Issue 1, pp. 427, (2016) (PubMed).

Qin, Lu, Guo, Luo, Hu, Huang, Chen, Wang, Ma, Yang, Jiang, Zhou: "A deleterious role for Th9/IL-9 in hepatic fibrogenesis." in: Scientific reports, Vol. 6, pp. 18694, (2016) (PubMed).

Huai, Dong, Zhu, Li, Cao, Gao, Yang, Wang, Mao: "Genetically Engineered Virus Nanofibers as an Efficient Vaccine for Preventing Fungal Infection." in: Advanced healthcare materials, Vol. 5, Issue 7, pp. 786-94, (2016) (PubMed).

Hassan, Wang, Xu, Yan, Song, Li: "DNA vaccination with a gene encoding Toxoplasma gondii Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice." in: Parasites & vectors, Vol. 7, pp. 431, (2015) (PubMed).

Zhang, Qian, Liu, Qian, Chen, Li: "Virus-like particles of chimeric recombinant porcine circovirus type 2 as antigen vehicle carrying foreign epitopes." in: Viruses, Vol. 6, Issue 12, pp. 4839-55, (2015) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Background:

Background: Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Gene Full Name: interferon gamma

Gene ID: 15978

UniProt: P01580

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy