IL-3 ELISA Kit
Quick Overview for IL-3 ELISA Kit (ABIN2648694)
Target
See all IL-3 ELISA KitsReactivity
Detection Method
Application
Sample Type
-
-
Purpose
- Human Interleukin 3 (IL-3) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 3 (IL-3) concentrations in cell culture supernates, serum, and plasma.
-
Analytical Method
- Quantitative
-
Sensitivity
- 15 pg/ml
-
Characteristics
- Human Interleukin 3 (IL-3) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
-
-
Interleukin 3 (IL-3) ELISA Kit
IL-3 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Chicken Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Cow Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Rat Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Dog Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Interleukin 3 (IL-3) ELISA KitSmall Sample IL-3 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Rat AA 27-169 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Serum
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Human AA 20-152 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Mouse AA 27-166 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Serum
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Sample Volume
- 100 μL
-
Assay Time
- 3.5 h
-
Plate
- Pre-coated
-
Protocol
- Human Interleukin 3 (IL-3) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-3 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-3 is added to the wells and binds to the combination of capture antibody- IL-3 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-3 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-3 standard dilutions and IL-3 sample concentration determined.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
-
- IL-3 (Interleukin 3 (IL-3))
-
Alternative Name
- IL-3
-
Pathways
- JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
Target See all IL-3 ELISA Kits
-
