IL-5 ELISA Kit

Catalog No. ABIN2648700

This Human IL-5 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IL-5.

Quick Overview for IL-5 ELISA Kit (ABIN2648700)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details IL-5 ELISA Kit

Purpose: Human Interleukin 5 (IL-5) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 5 (IL-5) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Sensitivity: 2 pg/ml

Characteristics: Human Interleukin 5 (IL-5) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: PRINCIPLE OF THE ASSAY Human Interleukin 5 (IL-5) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-5 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-5 is added to the wells and binds to the combination of capture antibody- IL-5 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-5 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-5 standard dilutions and IL-5 sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL-5

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour