IL12B ELISA Kit
Quick Overview for IL12B ELISA Kit (ABIN2648767)
Target
See all IL12B ELISA KitsReactivity
Detection Method
Application
Sample Type
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Purpose
- Human Interleukin 12p40 (IL-12p40) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 12p40 (IL-12p40) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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Sensitivity
- 30 pg/ml
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Characteristics
- Human Interleukin 12p40 (IL-12p40) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Interleukin 12 beta (IL12B) ELISA Kit
IL12B Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B Reactivity: Horse Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B Reactivity: Mouse AA 23-335 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 12 beta (IL12B) ELISA KitIL12B Reactivity: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Interleukin 12 beta (IL12B) ELISA KitIL12B Reactivity: Sheep Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B Reactivity: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B Reactivity: Chicken Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B Reactivity: Goat Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 12 beta (IL12B) ELISA KitIL12B Reactivity: Guinea Pig Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 100 μL
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Assay Time
- 3.5 h
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Plate
- Pre-coated
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Protocol
- Human Interleukin 12p40 (IL-12p40) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12p40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12p40 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-12p40 is added to the wells and binds to the combination of capture antibody- IL-12p40 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-12p40 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12p40 standard dilutions and IL-12p40 sample concentration determined.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- IL12B (Interleukin 12 beta (IL12B))
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Alternative Name
- IL-12p40
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Pathways
- JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Target See all IL12B ELISA Kits
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