CXCL2 ELISA Kit

Catalog No. ABIN2648785

Mouse CXCL2 ELISA Kit Colorimetric assay for quantification of Mouse CXCL2.

Quick Overview for CXCL2 ELISA Kit (ABIN2648785)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivity: Mouse

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details CXCL2 ELISA Kit

Sensitivity: 4 pg/ml

Characteristics: Mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2, was originally identified as a heparin-binding protein secreted by an LPS-stimulated mouse macrophage cell line (1). A cDNA clone encoding the protein was isolated from this cell line and characterized (2). Based on its protein and DNA sequences, mouse MIP-2 was classified as a member of the alpha (CXC) chemokine family of inflammatory and immunoregulatory cytokines (3). Mouse MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature mouse MIP-2. The protein sequence of mouse MIP-2 shows approximately 63 % identity to that of mouse KC, another mouse alpha chemokine. Mouse MIP-2 is also 60 % identical to human GROβ and GROγ(2). Based on these protein sequence similarities, it is likely that mouse KC and MIP-2 are homologs of human GROα, β and γ chemokines. Since chemokines with protein sequence homology to human IL-8 have not been identified in mice, it has been suggested that the mouse KC and MIP-2 are functional homologs of human IL-8 in mice (3, 4). A putative mouse homolog of the human IL-8 receptor beta (IL-8 Rβ) has also been cloned. This receptor shows 71 % identity to human IL-8 Rβ and 68 % identity to human IL-8 Rα. Both mouse KC and MIP-2 bind mouse IL-8 Rβ with high affinity (5). Like human IL-8, mouse MIP-2 exhibits potent neutrophil chemotactic activity and may be a key mediator of neutrophil recruitment in response to tissue injury and infection (3, 4). Increased MIP-2 expression has been found to be associated with neutrophil influx in various inflammatory conditions (6 - 10).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: Mouse Macrophage Inflammatory Protein 2 (MIP-2) ELISA Assay Kit employs quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for MIP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-2 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for MIP-2 is added to the wells and binds to the combination of capture antibody-MIP-2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of MIP-2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MIP-2 standard dilutions and MIP-2 sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: MIP-2

Pathways: Cellular Response to Molecule of Bacterial Origin