CXCL2 ELISA Kit

Catalog No. ABIN6719964

Product Details CXCL2 ELISA Kit

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: C-X-C motif chemokine 2, Chemokine (C-X-C motif) ligand 2, CINC-2a, Cxcl2, CXCL2_HUMAN, Gro beta, Gro-beta, GRO-beta(5-73), GRO-beta-T, GRO2, GRO2 oncogene, GROb, Growth-regulated protein beta, Hematopoietic synergistic factor, HSF, Macrophage inflammatory protein 2-alpha, Melanoma growth stimulatory activity beta, MGSA beta, MGSA-b, MIP 2, MIP 2a, MIP2, MIP2-alpha, MIP2A, SB-251353, SCYB2

Background: MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells.

Gene Name: CXCL2

Production: Natural and recombinant rat MIP-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: S32-N100

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat MIP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat MIP-2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: CXCL2 - MIP-2 (CXCL2 Products)

Synonyms: CINC-2a ELISA Kit, GRO2 ELISA Kit, GROb ELISA Kit, MGSA-b ELISA Kit, MIP-2a ELISA Kit, MIP2 ELISA Kit, MIP2A ELISA Kit, SCYB2 ELISA Kit, GRO3 ELISA Kit, Gro2 ELISA Kit, MIP-2 ELISA Kit, Mgsa-b ELISA Kit, Mip2 ELISA Kit, Scyb ELISA Kit, Scyb2 ELISA Kit, Mip-2 ELISA Kit, GROB ELISA Kit, MIP-2alpha ELISA Kit, C-X-C motif chemokine ligand 2 ELISA Kit, chemokine (C-X-C motif) ligand 2 ELISA Kit, CXCL2 ELISA Kit, Cxcl2 ELISA Kit

Gene ID: 114105

NCBI Accession: NP_446099

UniProt: P30348

Pathways: Cellular Response to Molecule of Bacterial Origin