Normetanephrine & Metanephrine ELISA Kit

Catalog No. ABIN2648796

The Chemical Normetanephrine & Metanephrine ELISA Kit (ABIN2648796) is a Colorimetric ELISA Kit designed to quantify Chemical Normetanephrine & Metanephrine.

Quick Overview for Normetanephrine & Metanephrine ELISA Kit (ABIN2648796)

Target: Normetanephrine & Metanephrine

Reactivity: Chemical

Detection Method: Colorimetric

Application: ELISA

Sample Type: Plasma (EDTA)

Product Details

Purpose: The Normetanephrine & Metanephrine (Plasma) ELISA assay kit provides materials for the quantitative measurement of free normetanephrine and metanephrine in EDTA plasma.

Analytical Method: Quantitative

Sensitivity: Metanephrine: 10 pg/ml, Normetanephrine: 15 pg/ml

Characteristics: Normetanephrine and metanephrine are physiologically formed from the catechol-amines noradrenaline and adrenaline by the enzyme catechol-O-methyltransferase (COMT). Increased levels of normetanephrine and metanephrine can be found in pheochromocytoma, ganglioneuroma and other neurogenic tumors. The Normetanephrine & Metanephrine (Plasma) ELISA assay kit provides materials for the quantitative measurement of free normetanephrine and metanephrine in EDTA plasma. Normetanephrine and metanephrine are quantitatively acylated to their N-acyl-derivates.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 400 μL

Protocol: The competitive Normetanephrine & Metanephrine (Plasma) ELISA Kit uses the microtiter plate format. Metanephrine and normetanephrine, respectively, are bound to the solid phase of the microtiter plate. Acylated nor-/metanephrines from the sample and solid phase bound nor-/metanephrines compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase nor-/metanephrine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Normetanephrine & Metanephrine