RFP Western Blot Kit: for RFP Chemiluminescent Western Blotting

Catalog No. ABIN2669872

Supplier Product No.: kca379

Quick Overview for RFP Western Blot Kit: for RFP Chemiluminescent Western Blotting (ABIN2669872)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Application: Dot Blot (DB), Western Blotting (WB)

Product Details

Clonality: Polyclonal

Supplier Product No.: kca379

Supplier: Rockland

Cross-Reactivity: Cherry, Tomato (Solanum lycopersicum), Banana, Orange, Plum, Strawberry

Application Details

Application Notes: Use Anti-RFP Chemiluminescent Kit for Western Blotting for detection of RFP-tagged recombinant proteins by western blot. his kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.

Comment:

Detection Kit Type: Chemiluminescent Western Blot Kit

Restrictions: For Research Use only

Handling

Handling Advice: Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!

Storage: RT/4 °C

Target Details

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: DsRed Fluorescent Protein

Background: Red Fluorescent Protein (RFP) Western Bloting kit allows for the detection of RFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with optimized Anti-RFP antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Synonyms: Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit, RFP, rRFP, mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry