Mouse TNF ELISpot Set

Catalog No. ABIN2691038

Quick Overview for Mouse TNF ELISpot Set (ABIN2691038)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Detection Method: Colorimetric

Application: ELISpot

Sample Type: Cell Samples

Product Details

Method Type: Cell ELISA

Brand: BD™ ELISPOT

Characteristics: The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro. Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). Unique strengths of the assay include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed biological samples, and compatibility with other assays. For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis.

Components: 10 ELISPOT plates
Unlabeled Capture Antibody
Biotinylated Detection Antibody
Enzyme Conjugate
Certificate of Analysis

Application Details

Application Notes: Unique strengths of the assay include the following:
High sensitivity
High throughput, high content analysis
Minimal volume of biological material required
Applicability to frozen/thawed biological samples
Compatibility with other assays. For example, cells analyzed by BD™ ELISPOT can be transferred for cloning, proliferation assays,flow cytometry, or other methods of analysis.

Comment:

BD™ ELISPOT Mouse TNF ELISPOT Set

Plate: Uncoated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store unopened reagents at 2-8°C. Do not use reagents after expiration date, or if turbidity is evident.

References

Helms, Boehm, Asaad, Trezza, Lehmann, Tary-Lehmann: "Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3723-32, (2000) (PubMed).

Power, Grand, Ismail, Peters, Yurkowski, Bretscher: "A valid ELISPOT assay for enumeration of ex vivo, antigen-specific, IFNgamma-producing T cells." in: Journal of immunological methods, Vol. 227, Issue 1-2, pp. 99-107, (1999) (PubMed).

Tary-Lehmann, Hricik, Justice, Potter, Heeger: "Enzyme-linked immunosorbent assay spot detection of interferon-gamma and interleukin 5-producing cells as a predictive marker for renal allograft failure." in: Transplantation, Vol. 66, Issue 2, pp. 219-24, (1998) (PubMed).

VanCott, Staats, Pascual, Roberts, Chatfield, Yamamoto, Coste, Carter, Kiyono, McGhee: "Regulation of mucosal and systemic antibody responses by T helper cell subsets, macrophages, and derived cytokines following oral immunization with live recombinant Salmonella." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 4, pp. 1504-14, (1996) (PubMed).

Fujihashi, McGhee, Beagley, McPherson, McPherson, Huang, Kiyono: "Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells." in: Journal of immunological methods, Vol. 160, Issue 2, pp. 181-9, (1993) (PubMed).

Czerkinsky, Andersson, Ekre, Nilsson, Klareskog, Ouchterlony: "Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells." in: Journal of immunological methods, Vol. 110, Issue 1, pp. 29-36, (1988) (PubMed).

Rönnblom, Cederblad, Sandberg, Alm: "Determination of herpes simplex virus-induced alpha interferon-secreting human blood leucocytes by a filter immuno-plaque assay." in: Scandinavian journal of immunology, Vol. 27, Issue 2, pp. 165-70, (1988) (PubMed).

Czerkinsky, Nilsson, Nygren, Ouchterlony, Tarkowski: "A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells." in: Journal of immunological methods, Vol. 65, Issue 1-2, pp. 109-21, (1984) (PubMed).

Sedgwick, Holt: "A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells." in: Journal of immunological methods, Vol. 57, Issue 1-3, pp. 301-9, (1983) (PubMed).

Target Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF