TNF alpha ELISA Kit

Catalog No. ABIN411363

The Mouse TNF alpha ELISA Kit (ABIN411363) is a Colorimetric ELISA Kit designed to quantify Mouse TNF alpha. This ELISA Kit has been cited in 179 publications.

Quick Overview for TNF alpha ELISA Kit (ABIN411363)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Binding Specificity: AA 80-235

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details TNF alpha ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich Mouse TNF Alpha/Tumor necrosis factor ELISA Kit to quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: L80-L235

Capture antibody and Detection antibody: monoclonal antibody from hamster, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.6%, 7.8%, 7.4%
Inter-Assay Precision (CV%): 5.7%, 8.3%, 8%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for TNF alpha ELISA Kit (ABIN411363)

Cai, Chen, Zhong, Zhang, Jiang, Xu, Shi: "Synergistic Effect of Compounds from a Chinese Herb: Compatibility and Dose Optimization of Compounds from N-Butanol Extract of Ipomoea stolonifera." in: Scientific reports, Vol. 6, pp. 27014, (2018) (PubMed).

Lin, Chen, Xia, Yang: "MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3." in: Oncotarget, Vol. 7, Issue 31, pp. 48978-48994, (2018) (PubMed).

Li, Jin, Wang, Li, Liu, Chang, Wang, Liu: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." in: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).

Pang, Wu, Peng, Yang, Kuai, Qin, Cao, Sun, Chen, Vitek, Jiang: "Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity." in: Oncotarget, Vol. 7, Issue 35, pp. 56030-56044, (2018) (PubMed).

Dong, Liu, Meng, Liu, Bi, Wu, Jin, Yao, Tang, Wang, Li, Zhang, Yu, Zhan, Chen, Ge, Yang, Li: "Keratin 8 limits TLR-triggered inflammatory responses through inhibiting TRAF6 polyubiquitination." in: Scientific reports, Vol. 6, pp. 32710, (2018) (PubMed).

Gao, Huang, Zhao, Hu, Li, Guo, Zhao, Lu: "LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling." in: Oncotarget, Vol. 7, Issue 39, pp. 63981-63994, (2018) (PubMed).

Bettaieb, Cremonini, Kang, Kang, Haj, Oteiza: "Anti-inflammatory actions of (-)-epicatechin in the adipose tissue of obese mice." in: The international journal of biochemistry & cell biology, Vol. 81, Issue Pt B, pp. 383-392, (2018) (PubMed).

Yang, Lai, Zheng, Kang, Xian, Wang, Shi, Meng, Li, Chen, Zhang: "A novel pH-controlled hydrogen sulfide donor protects gastric mucosa from aspirin-induced injury." in: Journal of cellular and molecular medicine, Vol. 21, Issue 10, pp. 2441-2451, (2018) (PubMed).

Hu, Shen, Wang, Zhao: "Liver X Receptor Agonist TO901317 Attenuates Paraquat-Induced Acute Lung Injury through Inhibition of NF-κB and JNK/p38 MAPK Signal Pathways." in: BioMed research international, Vol. 2017, pp. 4652695, (2018) (PubMed).

Hu, Fan, Li, Guan, Qu, Pei, Liu: "Bortezomib protects against dextran sulfate sodium‑induced ulcerative colitis in mice." in: Molecular medicine reports, Vol. 15, Issue 6, pp. 4093-4099, (2018) (PubMed).

Wang, Zhang, Yang, Zou, Wang, Liu, Liu, Zhang, Xiao, Wang: "Resveratrol protects against L-arginine-induced acute necrotizing pancreatitis in mice by enhancing SIRT1-mediated deacetylation of p53 and heat shock factor 1." in: International journal of molecular medicine, Vol. 40, Issue 2, pp. 427-437, (2018) (PubMed).

Dong, Han, Wang, Qin, Yang, Cao, Chen: "Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice." in: BMC gastroenterology, Vol. 17, Issue 1, pp. 82, (2018) (PubMed).

Zhang, Wu, Xu, He, Yang, Li, Li, Ao, Cheng, Jia: "The cystathionine β-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia." in: Brain, behavior, and immunity, Vol. 66, pp. 332-346, (2018) (PubMed).

Wang, Dong, Wang, Liu, Sun, Li, Lin, Geng, Jin, Li: "Adolescent escitalopram prevents the effects of maternal separation on depression- and anxiety-like behaviours and regulates the levels of inflammatory cytokines in adult male mice." in: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, Vol. 62, pp. 37-45, (2018) (PubMed).

Li, Diao, Guo, Huang, Yang, Feng, Yan, Ning, Zheng, Chen, Wu: "VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism." in: Nature communications, Vol. 8, Issue 1, pp. 1322, (2018) (PubMed).

Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." in: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Wang, Chen, Ni, Cheng, Jia, Zhen: "miRNA-3473b contributes to neuroinflammation following cerebral ischemia." in: Cell death & disease, Vol. 9, Issue 1, pp. 11, (2018) (PubMed).

Zhang, Yang, Li, Yang, Zhao, Wang, Wu: "Immunostimulatory activity of water-extractable polysaccharides from Cistanche deserticola as a plant adjuvant in vitro and in vivo." in: PLoS ONE, Vol. 13, Issue 1, pp. e0191356, (2018) (PubMed).

Wang, Zhao, Xin, Pan, Wang, Yang: "Effectiveness of lentivirus-mediated RNA interference targeting mouse tumor necrosis factor α in vitro and in vivo." in: Experimental and therapeutic medicine, Vol. 15, Issue 2, pp. 2134-2139, (2018) (PubMed).

Qin, Gao, Liu, Liu, Wang, Zou: "Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment." in: Oncology letters, Vol. 15, Issue 2, pp. 1630-1638, (2018) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF

Background:

Background: Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30 % amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

Gene Full Name: tumor necrosis factor

Gene ID: 21926

UniProt: P06804

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome