CD163 ELISA Kit
Quick Overview for CD163 ELISA Kit (ABIN2708128)
Target
See all CD163 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 625 pg/mL
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Purpose
- Sandwich ELISA for quantitative detection of Human CD163 concentrations.
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Analytical Method
- Quantitative
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Sensitivity
- 263 pg/mL
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Components
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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Material not included
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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CD163 (CD163) ELISA Kit
CD163 Reactivity: Human Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
CD163 (CD163) ELISA KitCD163 Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
CD163 (CD163) ELISA KitCD163 Reactivity: Monkey Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
CD163 (CD163) ELISA KitCD163 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 Reactivity: Pig Colorimetric Sandwich ELISA 93.75 ng/mL - 6000 ng/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 Reactivity: Human Colorimetric Sandwich ELISA 157-10000 pg/mL Cell Lysate, Plasma, Serum
CD163 (CD163) ELISA KitCD163 Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 100 μL
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Plate
- Pre-coated
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Protocol
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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Restrictions
- For Research Use only
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Handling Advice
- Avoid freeze/thaw cycles
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Storage
- 4 °C,-20 °C
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Storage Comment
- Shipped and store at 4°C for 6 months, store at -20°C for one year.
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Expiry Date
- 6-12 months
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- CD163
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Alternative Name
- CD163
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Background
- Alternative Names: M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163
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Gene ID
- 9332
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UniProt
- Q86VB7
Target See all CD163 ELISA Kits
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