SCGB1A1 ELISA Kit
Quick Overview for SCGB1A1 ELISA Kit (ABIN2708407)
Target
See all SCGB1A1 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 62.5 pg/mL
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Purpose
- Sandwich ELISA for quantitative detection of Human Uteroglobin concentrations.
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Analytical Method
- Quantitative
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Sensitivity
- 24 pg/mL
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Components
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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Material not included
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA Kit
SCGB1A1 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 Reactivity: Rat Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSmall Sample SCGB1A1 Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 Reactivity: Human AA 22-91 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 Reactivity: Human Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 100 μL
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Plate
- Pre-coated
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Protocol
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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Restrictions
- For Research Use only
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Handling Advice
- Avoid freeze/thaw cycles
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Storage
- 4 °C,-20 °C
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Storage Comment
- Shipped and store at 4°C for 6 months, store at -20°C for one year.
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Expiry Date
- 6-12 months
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- SCGB1A1 (Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1))
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Alternative Name
- Uteroglobin
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Background
- Alternative Names: CC10, CCSP, UGB, Uteroglobin, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, Secretoglobin family 1A member 1, Urinary protein 1, UP-1, UP1, Urine protein 1
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Gene ID
- 7356
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UniProt
- P11684
Target See all SCGB1A1 ELISA Kits
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