MADCAM1 ELISA Kit

Catalog No. ABIN2748882

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse MADCAM1.

Quick Overview for MADCAM1 ELISA Kit (ABIN2748882)

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details MADCAM1 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich ELISA for quantitative detection of Mouse MADCAM-1 concentrations.

Analytical Method: Quantitative

Sensitivity: 59 pg/mL

Components:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Material not included:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Restrictions: For Research Use only

Handling

Handling Advice: Avoid freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Shipped and store at 4°C for 6 months, store at -20°C for one year.

Expiry Date: 6-12 months

Target Details for MADCAM1

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Alternative Name: MADCAM-1

Background: Alternative Names: Mucosal addressin cell adhesion molecule 1, MAdCAM-1, hMAdCAM-1

Gene ID: 17123

UniProt: Q61826