alpha 2 Antiplasmin ELISA Kit

Catalog No. ABIN1568087

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat alpha 2 Antiplasmin.

Quick Overview for alpha 2 Antiplasmin ELISA Kit (ABIN1568087)

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.813-500 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details alpha 2 Antiplasmin ELISA Kit

Minimum Detection Limit: 7.813 ng/mL

Purpose: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of this index.

Cross-Reactivity (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 1.85 ng/mL

Components: Assay plate: 1×
Standard: 2×
Sample Diluent : 1 × 20 mL
Assay Diluent A: 1 × 10 mL
Assay Diluent B: 1 × 10 mL
Detection Reagent A: 1 × 120 µL
Detection Reagent B: 1 × 120 µL
Wash Buffer(25 x concentrate): 1 × 30 mL
Substrate: 1 × 10 mL
Stop Solution: 1 × 10 mL
Plate sealer for 96 wells: 5×
Instruction: 1×

Material not included: Microplate reader, Pipettes and pipette tips, EP tube, Deionized or distilled water.

Application Details

Sample Volume: 100 μL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handling Advice: The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance. 2. The kit should not be used beyond the expiration date on the kit label. 3. Do not mix or substitute reagents with those from other lots or sources. 4. If samples generate values higher than the highest standard, further dilute the samples with the Sample Diluent and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique,incubation time or temperature, and kit age can cause variation in binding.

Storage: 4 °C

Expiry Date: 12 months

Target Details for alpha 2 Antiplasmin

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Alternative Name: Alpha-2-Plasmin Inhibitor (a2PI)

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones