The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Analytical Method
Quantitative
Sensitivity
1.12ng/mL
Characteristics
human Cysteine Rich Secretory Protein LCCL Domain Containing Protein 2 (CRISPLD2) ELISA Kit
Components
Assay plate: 1× Standard: 2× Sample Diluent : 1 × 20 mL Assay Diluent A: 1 × 10 mL Assay Diluent B: 1 × 10 mL Detection Reagent A: 1 × 120 µL Detection Reagent B: 1 × 120 µL Wash Buffer(25 x concentrate): 1 × 30 mL Substrate: 1 × 10 mL Stop Solution: 1 × 10 mL Plate sealer for 96 wells: 5× Instruction: 1×
Material not included
Microplate reader, Pipettes and pipette tips, EP tube, Deionized or distilled water.
Optimal working dilution should be determined by the investigator.
Plate
Pre-coated
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level this index were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level this index were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%