Neuropilin 1 ELISA Kit

Catalog No. ABIN2859216

This Rat Neuropilin 1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat Neuropilin 1.

Quick Overview for Neuropilin 1 ELISA Kit (ABIN2859216)

Target: Neuropilin 1 (NRP1)

Binding Specificity: AA 22-854

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 780-50.000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details Neuropilin 1 ELISA Kit

Minimum Detection Limit: 780 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Neuropilin-1

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: F22-D854

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <20pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: F22-D854

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the neuropilin family.

Tissue Specificity: Found in the embryonic nervous system.

Plate: Pre-coated

Protocol: rat Neuropilin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neuropilin-1 has been precoated onto 96-well plates. Standards(NSO, F22-D854) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuropilin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Neuropilin-1 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL rat Neuropilin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat Neuropilin-1 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 8.3, Standard deviation: 0.315, CV(%): 3.8
• Sample 2: n=16, Mean(ng/ml): 21, Standard deviation: 0.987, CV(%): 4.7
• Sample 3: n=16, Mean(ng/ml): 30, Standard deviation: 1.62, CV(%): 5.4,
• Sample 1: n=24, Mean(ng/ml): 8.6, Standard deviation: 0.421, CV(%): 4.9
• Sample 2: n=24, Mean(ng/ml): 24, Standard deviation: 1.32, CV(%): 5.5
• Sample 3: n=24, Mean(ng/ml): 35, Standard deviation: 2.38, CV(%): 6.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for Neuropilin 1

Target: Neuropilin 1 (NRP1)

Alternative Name: NRP1

Background:

Protein Function: Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis (By similarity). .

Background: NRP1(Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.

Synonyms: Neuropilin-1,Vascular endothelial cell growth factor 165 receptor,CD304,Nrp1,

Full Gene Name: Neuropilin-1

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gene ID: 246331

UniProt: Q9QWJ9

Pathways: Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals