Biglycan ELISA Kit

Catalog No. ABIN2859228

This Human Biglycan ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Biglycan.

Quick Overview for Biglycan ELISA Kit (ABIN2859228)

Target: Biglycan (BGN)

Binding Specificity: AA 38-368

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10.000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate

Product Details Biglycan ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Biglycan

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: D38-K368

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: D38-K368

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Tissue Specificity: Found in several connective tissues, especially in articular cartilages.

Plate: Pre-coated

Protocol: human Biglycan ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Biglycan has been precoated onto 96-well plates. Standards(NSO, D38-K368) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Biglycan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Biglycan amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Biglycan standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Biglycan standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.62, Standard deviation: 0.06, CV(%): 3.7
• Sample 2: n=16, Mean(ng/ml): 2.91, Standard deviation: 0.157, CV(%): 5.4
• Sample 3: n=16, Mean(ng/ml): 5.38, Standard deviation: 0.264, CV(%): 4.9,
• Sample 1: n=24, Mean(ng/ml): 1.52, Standard deviation: 0.062, CV(%): 4.1
• Sample 2: n=24, Mean(ng/ml): 3.07, Standard deviation: 0.209, CV(%): 6.8
• Sample 3: n=24, Mean(ng/ml): 6.34, Standard deviation: 0.33, CV(%): 5.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for Biglycan

Target: Biglycan (BGN)

Alternative Name: BGN

Background:

Protein Function: May be involved in collagen fiber assembly. .

Background: Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.

Synonyms: Biglycan,Bone/cartilage proteoglycan I,PG-S1,BGN,SLRR1A,

Full Gene Name: Biglycan

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

Gene ID: 633

UniProt: P21810

Pathways: Glycosaminoglycan Metabolic Process