CXCL3 ELISA Kit

Catalog No. ABIN2859240

This Rat CXCL3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat CXCL3.

Quick Overview for CXCL3 ELISA Kit (ABIN2859240)

Target: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Binding Specificity: AA 33-101

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details CXCL3 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CXCL3

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: R33-S101

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: R33-S101

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: rat CXCL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL3 has been precoated onto 96-well plates. Standards(E.coli, R33-S101) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CXCL3 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat CXCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CXCL3 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 170, Standard deviation: 13.26, CV(%): 7.8
• Sample 2: n=16, Mean(pg/ml): 375, Standard deviation: 19.5, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 602, Standard deviation: 27.09, CV(%): 4.5,
• Sample 1: n=24, Mean(pg/ml): 161, Standard deviation: 13.36, CV(%): 8.3
• Sample 2: n=24, Mean(pg/ml): 352, Standard deviation: 22.53, CV(%): 6.4
• Sample 3: n=24, Mean(pg/ml): 593, Standard deviation: 27.87, CV(%): 4.7

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for CXCL3

Target: CXCL3 (Chemokine (C-X-C Motif) Ligand 3 (CXCL3))

Alternative Name: CXCL3

Background:

Protein Function: Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. .

Background: Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

Synonyms: C-X-C motif chemokine 3,Cytokine-induced neutrophil chemoattractant 2,CINC-2,Macrophage inflammatory protein 2-alpha/beta,MIP2-alpha/beta,Cxcl3,Cinc2,

Full Gene Name: C-X-C motif chemokine 3

Cellular Localisation: Secreted.

Gene ID: 171551

UniProt: Q10746

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy