ANGPTL3 ELISA Kit

Catalog No. ABIN2859241

Human ANGPTL3 ELISA Kit Colorimetric assay for quantification of Human ANGPTL3.

Quick Overview for ANGPTL3 ELISA Kit (ABIN2859241)

Target: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Binding Specificity: AA 17-460

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312-20.000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details ANGPTL3 ELISA Kit

Minimum Detection Limit: 312 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ANGPTL3

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: sf21
Immunogen sequence: S17-E460

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: S17-E460

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 1 fibrinogen C-terminal domain.

Tissue Specificity: Expressed principally in liver. Weakly expressed in kidney. .

Plate: Pre-coated

Protocol: human ANGPTL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANGPTL3 has been precoated onto 96-well plates. Standards(sf21, S17-E460) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ANGPTL3 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ANGPTL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ANGPTL3 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 3.2, Standard deviation: 0.144, CV(%): 4.5
• Sample 2: n=16, Mean(ng/ml): 5.8, Standard deviation: 0.273, CV(%): 4.7
• Sample 3: n=16, Mean(ng/ml): 10.3, Standard deviation: 0.299, CV(%): 2.9,
• Sample 1: n=24, Mean(ng/ml): 3.5, Standard deviation: 0.238, CV(%): 6.8
• Sample 2: n=24, Mean(ng/ml): 6.2, Standard deviation: 0.459, CV(%): 7.4
• Sample 3: n=24, Mean(ng/ml): 12.1, Standard deviation: 0.762, CV(%): 6.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for ANGPTL3

Target: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Alternative Name: ANGPTL3

Background:

Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

Synonyms: Angiopoietin-related protein 3,Angiopoietin-5,ANG-5,Angiopoietin-like protein 3,ANGPTL3,ANGPT5,UNQ153/PRO179,

Full Gene Name: Angiopoietin-related protein 3

Cellular Localisation: Secreted.

Gene ID: 27329

UniProt: Q9Y5C1