Relaxin 1 ELISA Kit

Catalog No. ABIN2859323

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Relaxin 1.

Quick Overview for Relaxin 1 ELISA Kit (ABIN2859323)

Target: Relaxin 1 (RLN1)

Binding Specificity: AA 26-53

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78-5000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin)

Product Details Relaxin 1 ELISA Kit

Minimum Detection Limit: 78 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Relaxin 1

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: E.coli, R162-Cys185(A)&K26-S53(B)

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: R162-Cys185(A)&K26-S53(B)

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Prostate. Not expressed in placenta, decidua or ovary. .

Plate: Pre-coated

Protocol: human Relaxin 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Relaxin 1 has been precoated onto 96-well plates. Standards(E.coli,R162-Cys185 (A) & K26-S53 (B)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Relaxin 1 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human Relaxin 1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Relaxin 1 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 596, Standard deviation: 29.20, CV(%): 4.9
• Sample 2: n=16, Mean(pg/ml): 1354, Standard deviation: 69.05, CV(%): 5.1
• Sample 3: n=16, Mean(pg/ml): 2415, Standard deviation: 130.41, CV(%): 5.4,
• Sample 1: n=24, Mean(pg/ml): 688, Standard deviation: 35.77, CV(%): 5.2
• Sample 2: n=24, Mean(pg/ml): 1542, Standard deviation: 92.52, CV(%): 6
• Sample 3: n=24, Mean(pg/ml): 2229, Standard deviation: 151.57, CV(%): 6.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for Relaxin 1

Target: Relaxin 1 (RLN1)

Alternative Name: RLN1

Background:

Protein Function: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.

Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to human chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

Synonyms: Prorelaxin H1,Relaxin B chain,Relaxin A chain,RLN1,

Full Gene Name: Prorelaxin H1

Cellular Localisation: Secreted.

Gene ID: 6013

UniProt: P04808