LGALS3BP ELISA Kit

Catalog No. ABIN2859325

Human LGALS3BP ELISA Kit Colorimetric assay for quantification of Human LGALS3BP.

Quick Overview for LGALS3BP ELISA Kit (ABIN2859325)

Target: LGALS3BP (Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP))

Binding Specificity: AA 19-585

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin)

Product Details LGALS3BP ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Galectin-3BP

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: NSO, V19-D585

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: V19-D585

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts. .

Plate: Pre-coated

Protocol: human Galectin-3BP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-3BP has been precoated onto 96-well plates. Standards(NSO,V19-D585) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-3BP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-3BP amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Galectin-3BP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Galectin-3BP standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 477, Standard deviation: 27.7, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 1541, Standard deviation: 92.46, CV(%): 6
• Sample 3: n=16, Mean(pg/ml): 2626, Standard deviation: 128.7, CV(%): 4.9,
• Sample 1: n=24, Mean(pg/ml): 527, Standard deviation: 32.15, CV(%): 6.1
• Sample 2: n=24, Mean(pg/ml): 1712, Standard deviation: 114.7, CV(%): 6.7
• Sample 3: n=24, Mean(pg/ml): 3015, Standard deviation: 168.84, CV(%): 5.6

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for LGALS3BP

Target: LGALS3BP (Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP))

Alternative Name: LGALS3BP

Background:

Protein Function: Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells. .

Background: Galectin-3-binding protein is a protein that in humans is encoded by the LGALS3BP gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cellâ€"cell and cellâ€"matrix interactions. Using fluorescence inâ€"situ hybridization, the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds to galectin 1.

Synonyms: Galectin-3-binding protein,Basement membrane autoantigen p105,Lectin galactoside-binding soluble 3-binding protein,Mac-2-binding protein,MAC2BP,Mac-2 BP,Tumor-associated antigen 90K,LGALS3BP,M2BP,

Full Gene Name: Galectin-3-binding protein

Cellular Localisation: Secreted . Secreted, extracellular space, extracellular matrix.

Gene ID: 3959

UniProt: Q08380