Syndecan 1 ELISA Kit

Catalog No. ABIN2859341

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Syndecan 1.

Quick Overview for Syndecan 1 ELISA Kit (ABIN2859341)

Target: Syndecan 1 (SDC1)

Binding Specificity: AA 23-254

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10.000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details Syndecan 1 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human SDC1/Syndecan-1

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: Q23-G254

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Q23-G254

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the syndecan proteoglycan family.

Plate: Pre-coated

Protocol: human Syndecan-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Syndecan-1 has been precoated onto 96-well plates. Standards(NSO, Q23-G254) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Syndecan-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Syndecan-1 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Syndecan-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Syndecan-1 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.23, Standard deviation: 0.048, CV(%): 3.9
• Sample 2: n=16, Mean(ng/ml): 3.25, Standard deviation: 0.15, CV(%): 4.6
• Sample 3: n=16, Mean(ng/ml): 5.87, Standard deviation: 0.299, CV(%): 5.1,
• Sample 1: n=24, Mean(ng/ml): 1.32, Standard deviation: 0.07, CV(%): 5.3
• Sample 2: n=24, Mean(ng/ml): 4.05, Standard deviation: 0.235, CV(%): 5.8
• Sample 3: n=24, Mean(ng/ml): 6.42, Standard deviation: 0.417, CV(%): 6.5

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for Syndecan 1

Target: Syndecan 1 (SDC1)

Alternative Name: SDC1

Background:

Protein Function: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

Background: Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane(type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.

Synonyms: Syndecan-1,SYND1,CD138,SDC1,SDC,

Full Gene Name: Syndecan-1

Cellular Localisation: Membrane, Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form.

Gene ID: 6382

UniProt: P18827

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism