RNA ChIP-IT Control Kit
Quick Overview for RNA ChIP-IT Control Kit (ABIN2866108)
Reactivity
Application
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Brand
- ChIP-IT®
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Specificity
- The RNA ChIP-IT Control Kit - Human is quality-control tested to demonstrate that the positive and negative control antibodies are effective in performing RNA-chromatin immunoprecipitation.
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Amyloid beta 1-40 (Abeta 1-40) ELISA KitAbeta 1-40 Reactivity: Human Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 Reactivity: Human Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitReactivity: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Mouse Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reactivity: Human AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 Reactivity: Human Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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HeLa cells were grown, fixed and used to prepare chromatin as described in the RNA ChIP-IT Kit manual. RNA-ChIP reactions were then performed using 10 μl of Suz12 antibody and 10 μl of control rabbit IgG (0.2 μg/μl). The immunoprecipitated RNA and the control input RNA were then used in RT-PCR using lincSFPQ primer mix. Kit Components: 50 μl Suz12 rabbit polyclonal antibody 100 μl rabbit IgG (0.2 μg/μl) 400 μl lincSFPQ primer mix
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Restrictions
- For Research Use only
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Storage
- -20 °C
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Storage Comment
- The RNA-ChIP Control Kit - Human components are shipped on dry ice. The lincSFPQ primer mix, the Suz12 antibody and the control rabbit IgG should be stored at -20°C. This product is guaranteed for 6 months from date of receipt under the correct storage conditions. Aliquot the antibodies to avoid exposing to multiple freeze-thaw cycles.
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Expiry Date
- 6 months
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