Global DNA Methylation - LINE-1 Kit
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- Specificity
- The Global DNA Methylation - LINE-1 Kit is tested for sensitivity in detecting changes in global DNA methylation levels in 100 ng genomic DNA extracted from HeLa cells that are untreated or treated with 0.05 μM 5-azacytidine, a known inhibitor of DNA methyltransferases (DNMTs).
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Amyloid beta 1-40 (Abeta 1-40) ELISA KitAbeta 1-40 Reactivity: Human Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 Reactivity: Human Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitReactivity: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Mouse Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reactivity: Human AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivity: Human Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
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- Comment
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The DNA is MseI digested and hybridized to the provided LINE-1 probe according to the product manual instructions for capture of the Long Interspersed Nucleotide Element 1 repeat elements of human genomic DNA. The provided methylated and non-methylated DNA standards are used to generate a standard curve for DNA quantification. The OD 450nm results of Lot 11115003 with a 2.5 minute developing time are shown in Figure 1. The data is then blanked and the net readings are graphed using a quadratic equation to generate a standard curve for quantification (Figure 2). Results show a reduction in global DNA methylation for the 5-azacytidine-treated HeLa cells as expected.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- Components can be stored at -20°C prior to first use, then we recommend storing the components at the temperatures listed in the manual.
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